USP 81 ANTIBIOTIC ZONE READER

TRINITY V3 USP 81 Antibiotic Zone Reader

TRINITY V3 digital imaging automates zone reading and calculations for the USP (United States Pharmacopeia) 81 cylinder-plate assay. This greatly improves the accuracy, speed and standardization of reading.

The TRINITY V3 Antibiotic Zone Reader eliminates all transcription and calculation errors, providing significant labor savings and standardization of testing. Regular software updates including new features are available via website download. TRINITY V3 requires no routine maintenance as it contains no motors or moving parts, only solid state digital electronics.

Workflow
After inserting a plate into the TRINITY V3 drawer, a color digital image on-screen is analyzed instantly with zone diameters displayed. The user reviews and confirms zone sizes. Threshold and sensitivity settings allow for precise, consistent reading of difficult plates. After all plates are read, calculations are displayed. Plate images may be saved and archived for future review.

Plate Layout
TRINITY V3 layout of samples on the plates and number of plates per set follow the USP 81 method. Testing can be incorporated into the laboratory routine without altering current manual methods, materials, organisms, and plate sample layout pattern. The user may adjust plate layout options.

Test Results
Test results can be printed, saved, and exported. The standard curve is plotted with the average of standard (known) concentrations. The plotted curve is shown to help visualize on-scale and off-scale test result values. A correlation coefficient, slope and intercept are also displayed. All calculated values are displayed on-screen. Unknown concentrations are calculated based on the standard regression formula derived from the standard curve.

TRINITY V3 includes Installation Qualification (IQ), Operation Qualification (OQ), Performance Qualification (PQ) validation documents. Learn more >

TRINITY V3 complies with 21 CFR Part 11 for electronic records. Learn more >

Peni cylinder dispensers and peni cylinders are available from Giles Scientific. Learn more >

USP 81 Reading Screen - Darkfield Lighting

USP 81 Reading Screen - Brightfield Lighting

Assay Reports & Logs

USP 81 Plate Results

USP 81 Results

USP 81 Standard Curve Graph

USP 81 Raw Zone Measurements

Help Menu & Assay Resources

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USP 81 Training PowerPoint

Giles Scientific created a detailed USP 81 PowerPoint training presentation. This is especially useful for labs new to this assay. Listed below are sample slides. If you would like a copy, complete the Information Request Form and ask for this presentation in 'Comments'.

TRINITY V3 Customer Testimonials - USP 81 Antibiotic Zone Reader

“Amazing customer service for TRINITY V3 USP <81> Antibiotic Zone Reader”

“The TRINITY V3 system has been a great addition to our laboratory. It has significantly reduced the time that it takes to process our tests. The validation of the TRINITY V3 system was easy and went smoothly with the help of its amazing customer service group. We were able to cater the software and reporting to serve our laboratory needs. Questions regarding any concerns or transition difficulties were handled in a very quick manner. This system is extremely user friendly! I don’t know how we ever managed without it.”

Christina Ferriño
Microbiologist
DPT Laboratories
San Antonio, TX, USA
TRINITY V3 customer since 2013

"Significant labor savings and simple validation with TRINITY V3"

"TRINITY V3 has been an excellent addition to our laboratory. The IQ, OQ, PQ qualification was easily completed within a few weeks. Since implementation, the efficiency in our laboratory has increased significantly in comparison to our old system. We no longer lose a whole day to reading plate assays.

One of the reasons the qualification was smooth was because of the excellent customer service. Any small bump along the way was answered immediately and thoroughly by a team of efficient and dedicated individuals. The team was always available to assist our individual needs."

Laura Olson
QC Manager
Pharmgate Animal Health, Omaha, NE, USA
TRINITY V3 customer since 2011

"Excellent products and technical customer support"

"It has been a pleasure working with Giles Scientific and their incredible team. They offer some of the best customer support I have ever experienced with a vendor. They are very quick to reply to customer queries and are also very helpful. The quality of their peni dispenser and peni cylinders is excellent. The peni dispenser works like magic in setting up antibiotic assays. The analysts running the assay are very pleased using the peni dispenser.

We currently purchased the TRINITY V3 and it has made the antibiotic assay work a lot faster. It was very easy to run the qualification on the equipment and the technical support was very prompt and helpful with answering all our questions. Purchasing this piece of equipment was a great investment for the lab. It has saved the analysts a tremendous amount of time and has increased productivity and efficiency."

Heather Crowell
Manager, Microbiology Laboratory
Underwriter Laboratories, Canton, MA, USA
TRINITY V3 customer since 2010

"Quality products and wonderful customer support from Giles Scientific”

"Our lab has recently started testing the USP <81> method with our CRMs and it would not have been as smooth and quick without the TRINITY V3, Giles Scientific Peni Dispenser, and their wonderful sales and technical support. The technology is amazingly fast at reading the zones that we cannot even imagine how much time it would take without their products and service. The technical support has been lightning fast when questions arise and are always happy to help.

We also make Microbiological CRMs and the TRINITY V3 colony counting application has saved me so much time in the lab. It is great saving the images and exporting the data I need for each new lot of CRMs. Both programs are very user friendly and easy to navigate. Great time-saving and quality products and service."

Whitney Richter
Microbiologist II
Sigma-Aldrich RTC LLC
Laramie, WY, USA
TRINITY V3 customer since 2013

"Extremely effective instrument for reading and analysis of USP <81> cylinder-plate assays"

"The TRINITY V3 Zone Reader is an extremely effective instrument for reading and analysis of USP <81> cylinder-plate assays. The software is very intuitive, and doesn’t require a steep learning curve for new analysts. The qualification documentation is very streamlined and easy to follow. Customer service is also very responsive and helpful when questions arise."

Paul Horton
Senior Microbiologist
TriRx Pharmaceutical Services
Huntsville, AL, USA
TRINITY V3 customer since 2019

"Accurate and efficient reading of USP assay plates"

"Our lab purchased TRINITY V3 from Giles Scientific to reduce the time required to read USP assay plates, as well as improving the accuracy and efficiency of reading. The service provided from the Giles Scientific team was excellent from start to finish, any queries were responded to very quickly and regular contact was made following purchase to ensure we had no problems. The installation and qualification of TRINITY V3 was straight forward, the software allowed us to tailor the calculations according to our laboratory needs. I would definitely recommend any laboratory conducting antibiotic assays to invest in a TRINITY V3."

Jill O'Brien
Encap Drug Delivery
Livingston, West Lothian, Scotland, UK
TRINITY V3 customer since 2014

“Simple IQ OQ PQ Validation & Excellent Customer Service for TRINITY V3”

"The TRINITY V3 Antibiotic Zone Reader is a great addition to our laboratory. TRINITY V3 has improved our antimicrobial assay efficiency. The system was easy to qualify. The IQ, OQ, and PQ Protocols were easy to follow and provided step-by-step guidance. The software is very user-friendly. I especially like that you can view the layout of your assay before you proceed with reading plates. The system has decreased the amount of time it takes our analysts to read assay plates. Our customer service contact at Giles Scientific responds to all questions effectively and in a timely manner. We are considering purchasing another unit in the near future along with the plate count software."

Krishaun N. Caldwell
Senior Microbiologist
Qualitest Pharmaceuticals
Huntsville, AL, USA
TRINITY V3 customer since 2012

"TRINITY V3: A Reliable Instrument for our USP Antibiotic Potency Assays"

"The TRINITY V3 is flexible and very reliable for our USP antibiotic potency assays. The optics produce high quality images and the software interface is easy to use and navigate. It allows for visual verification of the inhibition zones on screen. TRINITY V3 is also fully compliant with 21 CFR Part 11 requirements."

Tom Dougherty
Fresenius Kabi
Grand Island, NY, USA
TRINITY V3 customer since 2007

"TRINITY V3: Consistent results and superb technical support"

"TRINITY V3 is very compact, user friendly and provides consistent results for USP antibiotic assays. This saves a lot of labor and effort. The technical support is superb. I really enjoy working with the TRINITY V3 technical people."

Vinod Kumar
Microbiologist
Pharmaceutical International Inc.
Hunt Valley, MD, USA
TRINITY V3 customer since 2010

"From Fisher-Lilly Zone Reader to TRINITY V3"

"TRINITY V3 saves time in our antibiotics lab by reading the zones and doing the USP <81> calculations. I worked in an antibiotics lab at another job for 10 years and we read plates with a Fisher-Lilly Zone Reader and did calculations using calculators. I really like how TRINITY V3 performs the calculations for you. After entering the sample information, you have results."

Billy Covey
Microbiologist
Natureplex
Olive Branch, Mississippi
TRINITY V3 customer since 2014

“USP <81> Microbiological Assay of Antibiotics with TRINITY V3”

“Microbiological Assay of Antibiotics in animal feeds by the USP <81> method have been conducted by Indiana State Chemist Laboratories for many years. In 2005, we replaced the Fisher Lilly Zone Reader with the Giles TRINITY V3 System. We found the system to be easy to use, easy to determine the edge of zones of inhibition and faster to read the plates. Furthermore, we have never needed to repair the unit or replace any parts. We recommend the TRINITY V3 System to any laboratory involved with microbiological assays.“

Dr. Hussein Ragheb
Director, Indiana State Chemist Laboratories
West Lafayette, IN, USA
TRINITY V3 customer since 2005

"Great Customer Service and Support from Giles Scientific!"

"TRINITY V3 is a well-built and easy to use device that never let us down in three years. It also enables us to produce faster results on our USP antibiotic bioassay in animal feeds. Since we have had TRINITY V3 we have asked for minor adjustments specific to our needs. These demands were treated rapidly and efficiently and the customer service is great!"

Vanessa Marsan
Nutreco Canada
St-Hyacinthe, Quebec, Canada
TRINITY V3 customer since 2005

"Easy to use and helpful technical support"

"TRINITY V3 is an excellent investment for our laboratory. The implementation has greatly increased our productivity and efficiency for our USP assays. The technical support was very helpful during the process and all our specific needs are covered through specific custom adjustments. Working with the TRINITY V3 system is a real pleasure. This built in device is compact and very easy to use."

Julie Riviere
Jubilant Hollister Stier
Quebec, Canada
TRINITY V3 customer since 2012

"TRINITY V3: It is the best instrument for our USP/EP/JP antibiotic potency assay"

"TRINITY V3 is the best instrument for our antibiotics potency assays USP/EP and JP as well. The optics produce high quality images and the software very good for the detection and the measurements of the inhibition zones. TRINITY V3 is also fully compliant with 21 CFR Part 11 requirements."

Gyula Kardos
QC Microbiology Manager
Xellia Pharmaceuticals Ltd.
Budapest, Hungary
TRINITY V3 customer since 2017

"TRINITY V3 greatly advances our USP 81 capabilities and quality"

"TRINITY V3 has increased our laboratory efficiency immensely! From the IQ, OQ, PQ, to daily use, to customer support, we have had nothing but excellent experiences. We were able to customize the system to achieve our individual laboratory specifications, and when we had questions during validation or routine use, The TRINITY V3 support team were always available to answer efficiently and thoroughly. This technology has greatly advanced our capabilities and quality."

Microbiology Analyst
Alcami Corporation
St. Louis, MO, USA
TRINITY V3 customer since 2013

"Fast setup and validation of TRINITY V3 Zone Reader"

"It has been pleasure working with the Giles Scientific sales and service team as we implemented the TRINITY V3 zone reading system for our USP antibiotic potency assays. Their qualification documentation was very simple and straightforward, and the support we received has been great. The system was completely implemented in just a few weeks and we’ve had nothing but praise from the analysts in the lab. The TRINITY V3 system has been very reliable and much easier to use than our old system. We’re looking forward to many years of working with Giles Scientific."

Lab Manager
Michigan-Based Pharmaceutical Manufacturer, USA
TRINITY V3 customer since 2009

"TRINITY V3: User friendly system with consistent results"

"TRINITY V3 instantly reads and calculates the zones of inhibition for our USP and MYCO antibiotic potency tests. It has significantly reduced our labor time for this assay. The software is very user friendly system and provides consistent results for our microbiology lab. I have worked with Giles Scientific for several years and their service and support is excellent."

Carlos Flores
Pharmaceutical Manufacturer
Puerto Rico
TRINITY V3 customer since 2005